Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)


  • Product nameAnti-HSF1 (phospho S326) antibody [EP1713Y]
    See all HSF1 primary antibodies
  • Description
    Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)
  • Specificityab76076 detects HSF1 phosphorylated on Ser326.
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Ser326 of human HSF1.

  • Positive control
    • HeLa cell lysate treated with heat (44ºC). Human breast carcinoma and stomach tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/80.
ICC 1/250 - 1/500.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similaritiesBelongs to the HSF family.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Anti-HSF1 (phospho S326) antibody [EP1713Y] images

  • Overlay histogram showing HeLa cells stained with ab76076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76076, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical analysis of paraffin-embedded human stomach using ab76076, at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ab76076, at 1/100 dilution.
  • All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution

    Lane 1 : HeLa cell lysates (untreated)
    Lane 2 : HeLa cell lysates, treated with heat (44o C)

    Lysates/proteins at 10 µg per lane.

    Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size : 57 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)

References for Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

This product has been referenced in:
  • Tabe Y  et al. Ribosomal Biogenesis and Translational Flux Inhibition by the Selective Inhibitor of Nuclear Export (SINE) XPO1 Antagonist KPT-185. PLoS One 10:e0137210 (2015). Read more (PubMed: 26340096) »
  • Carpenter RL  et al. Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene N/A:N/A (2014). IHC ; Human . Read more (PubMed: 24469056) »

See all 12 Publications for this product

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Thank you for contacting us and bringing this to our attention.

We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
The samples we tested were brain, heart, kidney and spleen for each, in mouse and r...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (colon)
Loading amount 20 µg
Specification colon
Treatment heat shock
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Young Ju Yoon

Verified customer

Submitted Sep 04 2009