Overview

  • Product name
    Anti-HSF2 antibody [EPR1715Y]
    See all HSF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR1715Y] to HSF2
  • Specificity
    This antibody reacts with HSF2.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human HSF2.

  • Positive control
    • Hela cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR1715Y
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab52758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    • Sequence similarities
      Belongs to the HSF family.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock factor protein 2 antibody
      • Heat shock transcription factor 2 antibody
      • HSF 2 antibody
      • HSF2 antibody
      • HSF2_HUMAN antibody
      • HSTF 2 antibody
      see all

    Images

    • Anti-HSF2 antibody [EPR1715Y] (ab52758) at 1/500 dilution + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 60 kDa

    References

    This product has been referenced in:
    • Lamoliatte F  et al. Large-scale analysis of lysine SUMOylation by SUMO remnant immunoaffinity profiling. Nat Commun 5:5409 (2014). Read more (PubMed: 25391492) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab52758.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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