Accession number: NG 011433.The ACT3 primers can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. Gamma actin is a constitutively expressed gene. The primers are located in exon 6 of the gamma actin gene, close to the 3` end of the gene. Together with the HsACT1 and HsACT2 primers these primers can be used to determine the abundance of a modification (e.g. Histone H3 K4 mono methylation, see image with ab8895) or a factor (e.g. RNA polymerase II, see image with ab26721) over the gamma actin locus.
Our Abpromise guarantee covers the use of ab83627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Real Time PCR||Use at an assay dependent concentration.
Suitable for the analysis of chromatin immunoprecipitated DNA using Sybr® green real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.
ab83627 has not yet been referenced specifically in any publications.
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