Accession number: NG 011433. The HsACT1 primers can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells.
Gamma actin is a constitutively expressed gene. The primers are located around the core promoter of the gamma actin gene. In most cell types the gamma actin promoter is enriched with histone modifications and core promoter binding factors (e.g. TBP, see image with ab51841) associated with active gene transcription. Together with the HsACT2 and HsACT3 primers these primers can be used to determine the abundance of a modification (e.g. Histone H3 K4 mono methylation, see image with ab8895) or a factor (e.g. RNA polymerase II, see image with ab26721) over the gamma actin locus.
RelevanceActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates there are three main groups of actin isoforms, alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Defects in ACTG1 are the cause of non-syndromic sensorineural deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Real Time PCR
Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Sybr® green real time PCR.
Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.