HsGAPDH ChIP primer Exon 1 (Forward) (ab83588)

Overview

  • Product name
    HsGAPDH ChIP primer Exon 1 (Forward)
    See all GAPDH dna rna and cdna
  • General notes


    Accession number: NM_002046.
    The HsGAPDH primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is a highly expressed housekeeping gene. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types, this locus is highly enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab71604).

  • Tested applications
    Suitable for: Real Time PCRmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab83588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Real Time PCR Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

References

ab83588 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mou...

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Thank you for your reply. I have confirmed with our laboratory that the primer pairs were specifically generated for the human sequences.  We have not tested them with mouse and therefore cannot confirm that they give a single, specific PCR product...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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