HsGAPDH ChIP primer Exon 1 (Reverse) (ab83589)

Overview

  • Product name
    HsGAPDH ChIP primer Exon 1 (Reverse)
    See all GAPDH dna rna and cdna
  • General notes

    Accession number: NM_002046.The HsGAPDH primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is a highly expressed housekeeping gene. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types, this locus is highly enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab71604).

  • Tested applications
    Suitable for: Real Time PCRmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab83589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Real Time PCR Use at an assay dependent concentration.

Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

References

ab83589 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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