ab69549 detects an ~104 kDa protein, corresponding to the apparent molecular mass of Hsp104 on SDS-PAGE immunoblots, in samples from yeast (S. cerevisiae) origins. This antibody also detects an ~50 kDa band in yeast samples on immunoblot analysis. No reactivity is detected in immunoblot analysis with human, mouse, rat, monkey, and hamster proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000. Detects bands of approximately 104 & 50 kDa (predicted molecular weight: 102 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 is vital to protect yeast cells against high temperature and high concentration of ethanol. It is not required for normal growth.