Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp104
  • Specificity
    ab69549 detects an ~104 kDa protein, corresponding to the apparent molecular mass of Hsp104 on SDS-PAGE immunoblots, in samples from yeast (S. cerevisiae) origins. This antibody also detects an ~50 kDa band in yeast samples on immunoblot analysis. No reactivity is detected in immunoblot analysis with human, mouse, rat, monkey, and hamster proteins.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide:

    DDDNEDSMEIDDDLD

    conjugated to KLH, corresponding to amino acids 894-908 of Saccharomyces cerevisiae Hsp104

  • Positive control
    • S. cerevisiae cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/100.
    WB: 1/5000. Detects bands of approximately 104 & 50 kDa (predicted molecular weight: 102 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 is vital to protect yeast cells against high temperature and high concentration of ethanol. It is not required for normal growth.
    • Cellular localization
      Cytoplasmic and Nuclear
    • Database links
      • Alternative names
        • L0948 antibody
        • Heat shock protein 104 antibody
        • Hsp 104 antibody

      Images

      • All lanes : Anti-Hsp104 antibody (ab69549) at 1/1000 dilution

        Lane 1 : Molecular weight marker
        Lane 2 : Lysates prepared from saccharomyces cerevisiae


        Predicted band size : 102 kDa

      References

      This product has been referenced in:
      • Tsai CJ  et al. Hsp31 Is a Stress Response Chaperone That Intervenes in the Protein Misfolding Process. J Biol Chem 290:24816-34 (2015). Read more (PubMed: 26306045) »
      • O'Driscoll J  et al. Prion aggregate structure in yeast cells is determined by the Hsp104-Hsp110 disaggregase machinery. J Cell Biol 211:145-58 (2015). WB . Read more (PubMed: 26438827) »

      See all 4 Publications for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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