• Product nameAnti-Hsp105 antibody
    See all Hsp105 primary antibodies
  • Description
    Rabbit polyclonal to Hsp105
  • Specificityab53107 detects endogenous levels of total Hsp105 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide derived from human Hsp105.

  • Positive control
    • Human colon carcinoma tissue and HeLa cell extracts.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab53107 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab53107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/300 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • FunctionPrevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • Tissue specificityHighly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Post-translational
    Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Anti-Hsp105 antibody images

  • All lanes : Anti-Hsp105 antibody (ab53107) at 1/300 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • ab53107 at 1/50 dilution staining Hsp105 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
  • ICC/IF image of ab53107 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53107, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

References for Anti-Hsp105 antibody (ab53107)

ab53107 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The following reference reports HSP70 in the nuclei of cells at the onset of caspase activation: Nature. 2007 Jan 4;445(7123):102-5. Epub 2006 Dec 10. PMID: 17167422 and this reference describes the subcellular l...

Read More