Anti-Hsp105 antibody - C-terminal (ab166780)

Overview

  • Product name
    Anti-Hsp105 antibody - C-terminal
    See all Hsp105 primary antibodies
  • Description
    Rabbit polyclonal to Hsp105 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to the C terminal of Human Hsp105 (NP_006635.2).

  • Positive control
    • Human pancreas tissue; HeLa cell extract; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: without Mg2+, Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 96 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • Tissue specificity
    Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Post-translational
    modifications
    Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Hsp105 with ab166780 at 5 µg/ml.
  • All lanes : Anti-Hsp105 antibody - C-terminal (ab166780) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with synthesized peptide

    Predicted band size: 96 kDa

  • Immunofluorescent analysis of HeLa cells labeling Hsp105 with ab166780 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

References

ab166780 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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