Recombinant Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4576] to Hsp105/HSP110
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp105/HSP110 antibody [EPR4576]
See all Hsp105/HSP110 primary antibodies -
Description
Rabbit monoclonal [EPR4576] to Hsp105/HSP110 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, MCF-7, HeLa, C6, PC-12 and NIH/3T3 cell lysates and human cerebellum tissue lysate. ICC/IF: HeLa cells. IHC-P: Human thyroid gland carcinoma and liver carcinoma tissues. IP: NIH/3T3 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4576 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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IP | (1) |
1/10 - 1/100.
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IHC-P | (1) |
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities. -
Tissue specificity
Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10808 Human
- Entrez Gene: 15505 Mouse
- Entrez Gene: 288444 Rat
- Omim: 610703 Human
- SwissProt: Q92598 Human
- SwissProt: Q61699 Mouse
- SwissProt: Q66HA8 Rat
- Unigene: 36927 Human
see all -
Alternative names
- Antigen NY CO 25 antibody
- Antigen NY-CO-25 antibody
- DKFZp686M05240 antibody
see all
Images
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All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)
Lane 1 : C6 whole cell lysate
Lane 2 : PC-12 whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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ab109624 (purified) at 1/40 immunoprecipitating Hsp105/HSP110 in NIH/3T3 whole cell lysate.
Lane 1 (input): NIH/3T3 whole cell lysate (10µg)
Lane 2 (+): ab109624 + NIH/3T3 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109624 in NIH/3T3 whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human cerebellum lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 97 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab109624 has been referenced in 9 publications.
- Xu X et al. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. Redox Biol 59:102564 (2023). PubMed: 36473315
- Garofalo M et al. RNA Molecular Signature Profiling in PBMCs of Sporadic ALS Patients: HSP70 Overexpression Is Associated with Nuclear SOD1. Cells 11:N/A (2022). PubMed: 35053410
- Marcion G et al. Nanofitins targeting heat shock protein 110: An innovative immunotherapeutic modality in cancer. Int J Cancer 148:3019-3031 (2021). PubMed: 33506516
- Nosaka K et al. Heat Shock Protein 105 as an Immunotherapeutic Target for Patients With Cervical Cancer. Anticancer Res 41:4741-4751 (2021). PubMed: 34593423
- Liang Y et al. Activation of the IL-1ß/KLF2/HSPH1 pathway promotes STAT3 phosphorylation in alveolar macrophages during LPS-induced acute lung injury. Biosci Rep 40:N/A (2020). PubMed: 32091104
- Serlidaki D et al. Functional diversity between HSP70 paralogs caused by variable interactions with specific co-chaperones. J Biol Chem 295:7301-7316 (2020). PubMed: 32284329
- Deane CAS & Brown IR Differential Targeting of Hsp70 Heat Shock Proteins HSPA6 and HSPA1A with Components of a Protein Disaggregation/Refolding Machine in Differentiated Human Neuronal Cells following Thermal Stress. Front Neurosci 11:227 (2017). ICC/IF ; Human . PubMed: 28484369
- Deane CA & Brown IR Components of a mammalian protein disaggregation/refolding machine are targeted to nuclear speckles following thermal stress in differentiated human neuronal cells. Cell Stress Chaperones 22:191-200 (2017). PubMed: 27966060
- Cui Y & Gu X Proteomic changes of the porcine small intestine in response to chronic heat stress. J Mol Endocrinol 55:277-93 (2015). WB . PubMed: 26416815