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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
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Primary antibody notes ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.
Clone number J1G12
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
Use at an assay dependent dilution.
Function Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
Tissue specificity Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Sequence similarities Belongs to the heat shock protein 70 family.
Post-translational modifications Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
Cellular localization Cytoplasm.
Information by UniProt
Antigen NY CO 25 antibody
Antigen NY-CO-25 antibody
Anti-Hsp105 antibody [J1G12] images
Western blot - Hsp105 antibody [J1G12] (ab74174)
Anti-Hsp105 antibody [J1G12] (ab74174) at 1/1000 dilution + HeLa cell lysate at 30 µg Secondary Goat anti mouse antibody conjugated to HRP Developed using the ECL technique Predicted band size : 97 kDa Observed band size : 110 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Hsp105 antibody [J1G12] (ab74174) This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab74174 (1/200) staining Hsp105 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
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