Overview

  • Product name
    Anti-Hsp105 antibody [J1G12]
    See all Hsp105 primary antibodies
  • Description
    Mouse monoclonal [J1G12] to Hsp105
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human Hsp105 (1-858 aa) purified from E. coli

  • Positive control
    • HeLa cell lysate.
  • General notes


    ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Primary antibody notes
    ab74174, clone J1G12, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HSP105 protein.
  • Clonality
    Monoclonal
  • Clone number
    J1G12
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/200.

Target

  • Function
    Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • Tissue specificity
    Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Post-translational
    modifications
    Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Images

  • Anti-Hsp105 antibody [J1G12] (ab74174) at 1/1000 dilution + HeLa cell lysate at 30 µg

    Secondary
    Goat anti mouse antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 97 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab74174 (1/200) staining Hsp105 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

References

This product has been referenced in:
  • Zappasodi R  et al. Serological identification of HSP105 as a novel non-Hodgkin lymphoma therapeutic target. Blood 118:4421-30 (2011). WB ; Human . Read more (PubMed: 21860023) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted May 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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