The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 (or 0.2 µl/ml for 5 µg of Synechocystis PCC 6803 lysate) by ECL. Goat anti rabbit IgG:HRP was used as the secondary antibody. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Note: on a gradient gel this protein runs closer to 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HSP16.6 belongs to the class I family of small heat shock proteins. They are induced once a cell is stressed by an increase in temperature. Synechocystis Hsp16.6 is involved in the development of thermo tolerance and thylakoid stability. Small heat shock proteins appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Small heat-shock protein HSP16.6 antibody
Western blot - HSP16.6 antibody (ab80184)
All lanes : Anti-HSP16.6 antibody (ab80184) at 1/5000 dilution
Lane 1 : Total protein lysates from wild type cyanobacteria Lane 2 : HSP16.6 knocked protein lysates from cyanobacteria