Overview

  • Product name
    Anti-HSP16.6 antibody
  • Description
    Rabbit polyclonal to HSP16.6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with Synechocysis PCC 6803
  • Immunogen

    Recombinant protein. Gene accession number sII1514.

  • Positive control
    • Synechocystis PCC 6803 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/5000 (or 0.2 µl/ml for 5 µg of Synechocystis PCC 6803 lysate) by ECL. Goat anti rabbit IgG:HRP was used as the secondary antibody. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
    Note: on a gradient gel this protein runs closer to 11 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      HSP16.6 belongs to the class I family of small heat shock proteins. They are induced once a cell is stressed by an increase in temperature. Synechocystis Hsp16.6 is involved in the development of thermo tolerance and thylakoid stability. Small heat shock proteins appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
    • Alternative names
      • Small heat-shock protein HSP16.6 antibody

    Images

    • All lanes : Anti-HSP16.6 antibody (ab80184) at 1/5000 dilution

      Lane 1 : Total protein lysates from wild type cyanobacteria
      Lane 2 : HSP16.6 knocked protein lysates from cyanobacteria

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 17 kDa

    References

    ab80184 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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