Overview

  • Product name
    Anti-HSP17.6 antibody
  • Description
    Rabbit polyclonal to HSP17.6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
  • Immunogen

    Full length recombinant HSP17.6 protein produced in E. coli and purified by conventional methods (no affinity tag)

  • Positive control
    • Heat shocked (38° C for 2 hours) Arabidopsis thaliana lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

(ECL)

Target

  • Relevance
    HSP17.6 belongs to the class I family of small heat shock proteins. Small Hsps are induced when plant cells are stressed by increased temperature and appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are produced late in the seed maturation process.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • 17.6 kDa class I heat shock protein antibody
      • Arabidopsis thaliana heat shock protein 17.6A antibody
      • AT HSP17.6A antibody
      • F14F18.200 antibody
      • F14F18_200 antibody
      see all

    Images

    • Lanes 1, 2, 4, 5, 6 & 7 : Anti-HSP17.6 antibody (ab80183) at 1/1000 dilution
      Lane 3 :

      Lane 1 : Total protein from heat shocked Arabidopsis thaliana at 15 µg
      Lane 2 : Arabidopsis thaliana control plant
      Lane 3 : Empty lane
      Lane 4 : Recombinant purified HSP17.6, 1 ng
      Lane 5 : Recombinant purified HSP17.6, 2 ng
      Lane 6 : Lysates from Arabidopsis thaliana, 5 ng
      Lane 7 : recombinant purified HSP17.6, 10 ng

      Secondary
      Lanes 1, 2, 4, 5, 6 & 7 : HRP-conjugated goat anti rabbit IgG


      Predicted band size : 18 kDa

      15% SDS-PAGE and blotted 1hour onto nitrocellulose

    References

    ab80183 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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