Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp20
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Rat skeletal muscle Hsp20.

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab13491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa.
IP Use a concentration of 12.5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 23049607

Target

Images

  • Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
  • ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat ventricle tissue, staining Hsp20 with ab13491.

References

This product has been referenced in:
  • Hedegaard ER  et al. Mechanisms involved in increased sensitivity to adenosine A(2A) receptor activation and hypoxia-induced vasodilatation in porcine coronary arteries. Eur J Pharmacol 723:216-26 (2014). WB . Read more (PubMed: 24309216) »
  • Kirui DK  et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014). WB ; Human . Read more (PubMed: 24558362) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for contacting us with your question. The Hsp20 antibody ab13491 was raised against the full length protein isolated from rat skeletal muscle. Since the antibody is polyclonal, it should recognize all of the domains of the protein. I hope thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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