Overview

  • Product nameAnti-Hsp20 antibody
    See all Hsp20 primary antibodies
  • Description
    Rabbit polyclonal to Hsp20
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Hsp20.

    (Peptide available as ab88970.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; JEG-3; A549.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-Hsp20 antibody images

  • All lanes : Anti-Hsp20 antibody (ab68977) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • ICC/IF image of ab68977 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68977, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml.

References for Anti-Hsp20 antibody (ab68977)

ab68977 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 62.5 µg
Gel Running Conditions Reduced Denaturing (15% acrylamide)
Sample Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Specification Muscle sarcoplasmic extract
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shannon Cruzen

Verified customer

Submitted Feb 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"