The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at an assay dependent dilution.
Western Blot: Approx 20-25 kDa band seen in Human Muscle lysate [Predicted MW of approx. 22 kDa according to NP_055180].
Recommended for use at 0.5-1.0µg/ml.
ab4149 staining (1µg/ml) of Human Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-Hsp22 antibody (ab4149)
This product has been referenced in:
Sarparanta J et al. Mutations affecting the cytoplasmic functions of the co-chaperone DNAJB6 cause limb-girdle muscular dystrophy. Nat Genet44:450-5 (2012).
Read more (PubMed: 22366786) »
Laure L et al. Cardiac H11 kinase/Hsp22 stimulates oxidative phosphorylation and modulates mitochondrial reactive oxygen species production: Involvement of a nitric oxide-dependent mechanism. Free Radic Biol Med52:2168-2176 (2012).
Read more (PubMed: 22542467) »