Overview

  • Product name
  • Description
    Goat polyclonal to Hsp22
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NELPQDSQEVTCT

    , corresponding to C terminal amino acids 184-196 of Human Hsp22.

  • Positive control
    • Human Muscle lysate.
  • General notes
    Principal Names - H11; E2IG1; HSP22; HspB8; protein kinase H11; small stress protein-like protein HSP22 Official Gene Symbol - H11 (interim symbol) GenBank Accession Number – NP_055180 LocusLink ID - 26353 (human) Gene Ontology terms - protein serine/threonine kinase activity; heat shock protein activity; biological_process unknown; cellular_component unknown; transferase activity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20-25 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human Hsp22 peptide (ab22992).

Approx 20-25 kDa band seen in Human Muscle lysate [Predicted MW of approx. 22 kDa according to NP_055180].

ICC Use at an assay dependent concentration.

Target

  • Function
    Displays temperature-dependent chaperone activity.
  • Tissue specificity
    Predominantly expressed in skeletal muscle and heart.
  • Involvement in disease
    Neuronopathy, distal hereditary motor, 2A
    Charcot-Marie-Tooth disease 2L
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha crystallin C chain antibody
    • Alpha-crystallin C chain antibody
    • Charcot Marie Tooth disease axonal type 2L antibody
    • Charcot Marie Tooth disease spinal antibody
    • CMT2L antibody
    • CRYAC antibody
    • DHMN 2 antibody
    • DHMN2 antibody
    • E2 induced gene 1 protein antibody
    • E2-induced gene 1 protein antibody
    • E2IG1 antibody
    • H11 antibody
    • Heat shock 22kDa protein 8 antibody
    • Heat shock 27kDa protein 8 antibody
    • Heat shock protein 22 antibody
    • Heat shock protein beta 8 antibody
    • Heat shock protein beta-8 antibody
    • Hereditary motor neuropathy distal antibody
    • HMN 2 antibody
    • HMN2 antibody
    • HMN2A antibody
    • HSB8 antibody
    • HSPB 8 antibody
    • HspB8 antibody
    • HSPB8_HUMAN antibody
    • OTTHUMP00000239768 antibody
    • Protein kinase H11 antibody
    • Small stress protein like protein HSP22 antibody
    • Small stress protein-like protein HSP22 antibody
    • Spinal muscular atrophy distal adult autosomal dominant antibody
    see all

Images

  • ab4149 staining (1µg/ml) of Human Muscle lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab4149 staining (1µg/ml) of Human Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Sarparanta J  et al. Mutations affecting the cytoplasmic functions of the co-chaperone DNAJB6 cause limb-girdle muscular dystrophy. Nat Genet 44:450-5 (2012). Read more (PubMed: 22366786) »
  • Laure L  et al. Cardiac H11 kinase/Hsp22 stimulates oxidative phosphorylation and modulates mitochondrial reactive oxygen species production: Involvement of a nitric oxide-dependent mechanism. Free Radic Biol Med 52:2168-2176 (2012). WB . Read more (PubMed: 22542467) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 22 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2016

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
60 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Mr. Jörg Hoehfeld

Verified customer

Submitted Jun 27 2007

Thank you for taking the time to contact me regarding your experiences using this product, I am sorry to hear that you have been experiencing problems using this product in Western blotting. The working dilution (assuming a start conc. of 0.5mg/ml) ran...

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Thank you for submitting the completed Western Blot Questionnaire Form. We are very sorry to hear that you are having problem with this antibody. We would like to make the following comments/suggestions: 1. This antibody has been tested and char...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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