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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Hsp22 is approximately 0.03ng/ml as a capture antibody.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Displays temperature-dependent chaperone activity.
Tissue specificity Predominantly expressed in skeletal muscle and heart.
Involvement in disease Neuronopathy, distal hereditary motor, 2A Charcot-Marie-Tooth disease 2L
Sequence similarities Belongs to the small heat shock protein (HSP20) family.
Cellular localization Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
Information by UniProt
Alpha crystallin C chain antibody
Alpha-crystallin C chain antibody
Charcot Marie Tooth disease axonal type 2L antibody
Anti-Hsp22 antibody images
Western blot - Hsp22 antibody (ab66063)
All lanes : Anti-Hsp22 antibody (ab66063) at 1 µg/ml Lane 1 : Hsp22 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 50 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution Predicted band size : 22 kDa Observed band size : 25 kDa ( why is the actual band size different from the predicted?)
References for Anti-Hsp22 antibody (ab66063)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"