Overview

  • Product nameAnti-Hsp22 antibody
    See all Hsp22 primary antibodies
  • Description
    Mouse monoclonal to Hsp22
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment with tag: KVCVNVHSFK PEELMVKTKD GYVEVSGKHE EKQQEGGIVS KNFTKKIQLP AEVDPVTVFA SLSPEGLLII EAPQVPPYST FGESSFNNEL PQDSQEVTCT, corresponding to amino acids 97-197 of Human Hsp22

  • Positive control
    • Hsp22 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged Hsp22 is approximately 0.03ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDisplays temperature-dependent chaperone activity.
    • Tissue specificityPredominantly expressed in skeletal muscle and heart.
    • Involvement in diseaseNeuronopathy, distal hereditary motor, 2A
      Charcot-Marie-Tooth disease 2L
    • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
    • Cellular localizationCytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha crystallin C chain antibody
      • Alpha-crystallin C chain antibody
      • Charcot Marie Tooth disease axonal type 2L antibody
      • Charcot Marie Tooth disease spinal antibody
      • CMT2L antibody
      • CRYAC antibody
      • DHMN 2 antibody
      • DHMN2 antibody
      • E2 induced gene 1 protein antibody
      • E2-induced gene 1 protein antibody
      • E2IG1 antibody
      • H11 antibody
      • Heat shock 22kDa protein 8 antibody
      • Heat shock 27kDa protein 8 antibody
      • Heat shock protein 22 antibody
      • Heat shock protein beta 8 antibody
      • Heat shock protein beta-8 antibody
      • Hereditary motor neuropathy distal antibody
      • HMN 2 antibody
      • HMN2 antibody
      • HMN2A antibody
      • HSB8 antibody
      • HSPB 8 antibody
      • HspB8 antibody
      • HSPB8_HUMAN antibody
      • OTTHUMP00000239768 antibody
      • Protein kinase H11 antibody
      • Small stress protein like protein HSP22 antibody
      • Small stress protein-like protein HSP22 antibody
      • Spinal muscular atrophy distal adult autosomal dominant antibody
      see all

    Anti-Hsp22 antibody images

    • All lanes : Anti-Hsp22 antibody (ab66063) at 1 µg/ml

      Lane 1 : Hsp22 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 22 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    References for Anti-Hsp22 antibody (ab66063)

    ab66063 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Embryonic Heart)
    Permeabilization Yes - Tween-20
    Specification Embryonic Heart
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 22 2016

    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"