Overview

  • Product nameAnti-Hsp22 antibody
    See all Hsp22 primary antibodies
  • Description
    Rabbit polyclonal to Hsp22
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 134 and 196 of Human Hsp22.

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate. ICC/IF: HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionDisplays temperature-dependent chaperone activity.
  • Tissue specificityPredominantly expressed in skeletal muscle and heart.
  • Involvement in diseaseNeuronopathy, distal hereditary motor, 2A
    Charcot-Marie-Tooth disease 2L
  • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
  • Cellular localizationCytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha crystallin C chain antibody
    • Alpha-crystallin C chain antibody
    • Charcot Marie Tooth disease axonal type 2L antibody
    • Charcot Marie Tooth disease spinal antibody
    • CMT2L antibody
    • CRYAC antibody
    • DHMN 2 antibody
    • DHMN2 antibody
    • E2 induced gene 1 protein antibody
    • E2-induced gene 1 protein antibody
    • E2IG1 antibody
    • H11 antibody
    • Heat shock 22kDa protein 8 antibody
    • Heat shock 27kDa protein 8 antibody
    • Heat shock protein 22 antibody
    • Heat shock protein beta 8 antibody
    • Heat shock protein beta-8 antibody
    • Hereditary motor neuropathy distal antibody
    • HMN 2 antibody
    • HMN2 antibody
    • HMN2A antibody
    • HSB8 antibody
    • HSPB 8 antibody
    • HspB8 antibody
    • HSPB8_HUMAN antibody
    • OTTHUMP00000239768 antibody
    • Protein kinase H11 antibody
    • Small stress protein like protein HSP22 antibody
    • Small stress protein-like protein HSP22 antibody
    • Spinal muscular atrophy distal adult autosomal dominant antibody
    see all

Anti-Hsp22 antibody images

  • IHC image of Hsp22 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96837, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Hsp22 antibody (ab96837) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 22 kDa
    12% SDS PAGE
  • Immunofluorescence analysis of Hsp22 expression in PFA-fixed HeLa cells, using ab96837 at 1/200 dilution. Lower image was merged with DNA probe.

References for Anti-Hsp22 antibody (ab96837)

ab96837 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Zebrafish Embryo (muscle tissue)
Specification muscle tissue
Username

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Verified customer

Submitted Oct 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"