Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)


  • Product nameAnti-Hsp27 (phospho S15) antibody [EP2293Y]
    See all Hsp27 primary antibodies
  • Description
    Rabbit monoclonal [EP2293Y] to Hsp27 (phospho S15)
  • Specificityab76313 will detect Hsp27 phosphorylation on Serine 15.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human Hsp27.

  • Positive control
    • HeLa cells, HeLa cell lysate heat treated at 44°C.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 23 kDa.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionInvolved in stress resistance and actin organization.
    • Tissue specificityDetected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
    • Involvement in diseaseDefects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
      Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
    • Post-translational
      Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock 27kDa protein antibody
      • 28 kDa heat shock protein antibody
      • CMT2F antibody
      • DKFZp586P1322 antibody
      • epididymis secretory protein Li 102 antibody
      • Estrogen regulated 24 kDa protein antibody
      • Estrogen-regulated 24 kDa protein antibody
      • Heat shock 25kDa protein 1 antibody
      • Heat shock 27 kDa protein antibody
      • Heat shock 27kD protein 1 antibody
      • Heat shock 27kDa protein 1 antibody
      • Heat shock 28kDa protein 1 antibody
      • Heat Shock Protein 27 antibody
      • Heat shock protein beta 1 antibody
      • Heat shock protein beta-1 antibody
      • heat shock protein family B (small) member 1 antibody
      • HEL-S-102 antibody
      • HMN2B antibody
      • HS.76067 antibody
      • Hsp 25 antibody
      • HSP 27 antibody
      • Hsp 28 antibody
      • Hsp B1 antibody
      • Hsp25 antibody
      • HSP27 antibody
      • Hsp28 antibody
      • HspB1 antibody
      • HSPB1_HUMAN antibody
      • SRP27 antibody
      • Stress responsive protein 27 antibody
      • Stress-responsive protein 27 antibody
      see all

    Anti-Hsp27 (phospho S15) antibody [EP2293Y] images

    • All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313) at 1/2500 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
      Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 23 kDa
      Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
    • ab76313 staining Hsp27 (phospho S15) in HeLa (human cervix adenocarcinoma) cells with heat treatment at 44°C. by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab76313 (1/1000) and secondary antibody ab150077 (1/1000) respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    References for Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

    This product has been referenced in:
    • Tate CM  et al. LY2228820 dimesylate, a selective inhibitor of p38 mitogen-activated protein kinase, reduces angiogenic endothelial cord formation in vitro and in vivo. J Biol Chem 288:6743-53 (2013). WB . Read more (PubMed: 23335506) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa Cells)
    Loading amount 20 µg
    Specification HeLa Cells
    Gel Running Conditions Non-reduced Denaturing (12.5% gel)
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

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    Submitted Feb 01 2012