Recombinant
RabMAb

Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

Overview

  • Product name
    Anti-Hsp27 (phospho S82) antibody [EPR7278]
    See all Hsp27 primary antibodies
  • Description
    Rabbit monoclonal [EPR7278] to Hsp27 (phospho S82)
  • Host species
    Rabbit
  • Specificity
    ab155987 only detects Hsp27 phosphorylated at Serine 82.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hsp27. The exact sequence is proprietary.

  • Positive control
    • HeLa cell lysate treated with heat shock (44°C); Human skeletal muscle and urinary bladder transitional carcinoma tissues.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 23 kDa.

For unpurified, use 1/200.

IP 1/75.

For unpurified, use 1/10.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Involved in stress resistance and actin organization.
    • Tissue specificity
      Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
    • Involvement in disease
      Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
      Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    • Sequence similarities
      Belongs to the small heat shock protein (HSP20) family.
    • Post-translational
      modifications
      Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock 27kDa protein antibody
      • 28 kDa heat shock protein antibody
      • CMT2F antibody
      • DKFZp586P1322 antibody
      • epididymis secretory protein Li 102 antibody
      • Estrogen regulated 24 kDa protein antibody
      • Estrogen-regulated 24 kDa protein antibody
      • Heat shock 25kDa protein 1 antibody
      • Heat shock 27 kDa protein antibody
      • Heat shock 27kD protein 1 antibody
      • Heat shock 27kDa protein 1 antibody
      • Heat shock 28kDa protein 1 antibody
      • Heat Shock Protein 27 antibody
      • Heat shock protein beta 1 antibody
      • Heat shock protein beta-1 antibody
      • heat shock protein family B (small) member 1 antibody
      • HEL-S-102 antibody
      • HMN2B antibody
      • HS.76067 antibody
      • Hsp 25 antibody
      • HSP 27 antibody
      • Hsp 28 antibody
      • Hsp B1 antibody
      • Hsp25 antibody
      • HSP27 antibody
      • Hsp28 antibody
      • HspB1 antibody
      • HSPB1_HUMAN antibody
      • SRP27 antibody
      • Stress responsive protein 27 antibody
      • Stress-responsive protein 27 antibody
      see all

    Images

    • All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)


      Exposure time: 1 minute


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Dot blot analysis of Lane 1: Hsp27 (pS82) phospho peptide and Lane 2: Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 10 seconds.

    • All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified)

      Lane 1 : Mouse heart
      Lane 2 : Rat heart

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified) + HeLa cell lysate treated with heat shock (44°C) at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified)

      Lane 1 : Mouse heart
      Lane 2 : Rat heart

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified) + HeLa cells treated with heat shock (44°C) at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution (unpurified)

      Lane 1 : Untreated HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with heat shock (44°C).

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 23 kDa

    • ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    This product has been referenced in:
    • Rao W  et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis N/A:N/A (2014). Read more (PubMed: 24667688) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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