Synthetic phosphopeptide derived from a region of mouse HSP25 that contains serine 86.
Our Abpromise guarantee covers the use of ab17938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-Fr||Use at an assay dependent concentration. See Abreview.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
Hsp27 (phospho S86) antibody image 6534.
Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
Lane 1: unstimulated cells
Lane 2: cells stimulated with anisomycin
Lane 3: cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
Lane 4: cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
Lane 5: cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
Lane 6: cells stimulated with anisomycin and treated with lambda phosphatase
Only the phospho peptide immunogen blocks the antibody, confirming its specificity for the phospho epitope serine 86. The signal was completely removed by phosphatase treatment demonstrating that the antibody interacts specifically with the phosphorylated protein.