Overview

  • Product nameAnti-Hsp40 antibody [3B9.E6]
    See all Hsp40 primary antibodies
  • Description
    Mouse monoclonal [3B9.E6] to Hsp40
  • Specificityab78437 does not cross-react with Hdj2 or Ydj1.
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant Human Hsp40

  • Positive control
    • HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number3B9.E6
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInteracts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
  • Sequence similaritiesContains 1 J domain.
  • Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • Information by UniProt
  • Database links
  • Alternative names
    • DnaJ (Hsp40) homolog subfmaily B member 1 antibody
    • DNAJ 1 antibody
    • DNAJ B1 antibody
    • DnaJ homolog subfamily B member 1 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJ1 antibody
    • DNAJB 1 antibody
    • Dnajb1 antibody
    • DNAJB1 protein antibody
    • DNJB1_HUMAN antibody
    • HDJ 1 antibody
    • HDJ-1 antibody
    • HDJ1 antibody
    • Heat shock 40 kDa protein 1 antibody
    • Heat shock 40kD protein 1 antibody
    • Heat shock protein 40 antibody
    • Hsp 40 antibody
    • HSP40 antibody
    • HSPF 1 antibody
    • HSPF1 antibody
    • Human DnaJ protein 1 antibody
    • Radial spoke 16 homolog B antibody
    • RSPH16B antibody
    • Sis1 antibody
    see all

Anti-Hsp40 antibody [3B9.E6] images

  • Overlay histogram showing HeLa cells stained with ab78437 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78437, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Hsp40 antibody [3B9.E6] (ab78437)

This product has been referenced in:
  • Jo S  et al. Neuronal hypoxia induces hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism. J Neurosci 32:8491-500 (2012). Rat . Read more (PubMed: 22723689) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab78437.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"