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Recombinant human Hsp40
Our Abpromise guarantee covers the use of ab69402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||1/100 - 1/400.|
ab69402 staining Hsp40 in the A549 human lung cancer cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. A CF568-conjugated Donkey anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.
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