Overview

  • Product nameAnti-Hsp40 antibody
    See all Hsp40 primary antibodies
  • Description
    Rabbit polyclonal to Hsp40
  • SpecificityThis antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fish, Monkey
    Does not react with: Escherichia coli
  • Immunogen

    Recombinant human Hsp40

  • Positive control
    • HeLa heat shocked cell lysate and recombinant human Hsp40 Protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP 1/100.
ICC/IF 1/100 - 1/400.

Target

Anti-Hsp40 antibody images

  • All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Hsp40 recombinant protein
    Lane 3 : DnaJ recombinant protein
    Lane 4 : Cell lysates prepared from heat shocked hela cells
    Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
    Lane 6 : Cell lysates prepared from PC-12 cells


    Predicted band size : 38 kDa
  • ab69402 staining Hsp40 in the A549 human lung cancer cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. A CF568-conjugated Donkey anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Hsp40 antibody (ab69402)

This product has been referenced in:
  • Weber TA  et al. APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria. PLoS One 8:e63683 (2013). WB ; Human . Read more (PubMed: 23704930) »
  • Maffei S  et al. Beneficial effect of directional freezing on in vitro viability of cryopreserved sheep whole ovaries and ovarian cortical slices. Hum Reprod N/A:N/A (2013). IHC-Fr ; Sheep . Read more (PubMed: 24135077) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cell)
Specification A549 Lung cancer cell
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Jun 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"