The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa.
Use a concentration of 5 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionBinds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Involvement in diseaseNote=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
Sequence similaritiesBelongs to the serpin family.
serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
Serine or cysteine proteinase inhibitor clade H member 1 antibody
Serine or cysteine proteinase inhibitor clade H member 2 antibody
Serpin H1 antibody
Serpin peptidase inhibitor clade H member 1 antibody
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
Serpin peptidase inhibitor, clade H, member 1 antibody
Anti-Hsp47 antibody images
Flow Cytometry - Anti-Hsp47 antibody (ab54874)
Overlay histogram showing HepG2 cells stained with ab54874 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54874, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Hsp47 antibody (ab54874)
Predicted band size : 46 kDa Hsp47 antibody (ab54874) at 1ug/lane + HeLa cell lysate at 25ug/lane.
ICC/IF image of ab54874 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54874, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Hsp47 antibody (ab54874)
has not yet been referenced specifically in any publications.
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