Overview

  • Product nameAnti-Hsp60 antibody
    See all Hsp60 primary antibodies
  • Description
    Goat polyclonal to Hsp60
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey
  • Immunogen

    Recombinant protein (Human)

  • Positive control
    • WB: HeLa, NIH 3T3 or RK-13 cell lysate. IHC-P: adrenal, spleen or uterus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab109873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 61 kDa.
IP 1/100.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody images

  • All lanes : Anti-Hsp60 antibody (ab109873) at 1/1000 dilution

    Lane 1 : NSP-540-Hsp60 active recombinant protein
    Lane 2 : SSP-610-GroEL active recombinant protein
    Lane 3 : NSP-581-Hsp65 active recombinant protein
    Lane 4 : HeLa cell lysate
    Lane 5 : NIH 3T3 cell lysate
    Lane 6 : RK-13 cell lysate


    Predicted band size : 61 kDa
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human adrenal tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human spleen tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109873 at 10 µg/ml staining Hsp60 in formalin-fixed, paraffin-embedded Human uterus tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    After incubation with the primary antibody, slides were incubated with a biotinylated anti-Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-Hsp60 antibody (ab109873)

This product has been referenced in:
  • Liu K  et al. Phosphorylated AKT inhibits the apoptosis induced by DRAM-mediated mitophagy in hepatocellular carcinoma by preventing the translocation of DRAM to mitochondria. Cell Death Dis 5:e1078 (2014). ICC/IF ; Human . Read more (PubMed: 24556693) »

See 1 Publication for this product

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