Overview

  • Product nameAnti-Hsp60 antibody
    See all Hsp60 primary antibodies
  • Description
    Rabbit polyclonal to Hsp60
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Chicken, Pufferfish (Fugu), Zebrafish, Sea Urchin, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human Hsp60 aa 31-50.
    Sequence:

    KFGADARALMLQGVDLLADA

  • Positive control
    • Hela cells, NIH3T3 cells, Colon carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P 1/200.

Target

  • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody images

  • All lanes : Anti-Hsp60 antibody (ab31115) at 1/8000 dilution

    Lane 1 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
    Lane 2 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
    Lane 3 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
    Lane 4 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1
    Lane 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
    Lane 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
    Lane 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
    Lane 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1

    Lysates/proteins at 40 µg per lane.

    Secondary
    ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    Treatment for 30 minutes (lanes 1-4) or 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).

    See Abreview

  • Ab31115, at a 1/200 dilution, staining Hsp60 in formalin fixed, paraffin embedded human colon carcinoma tissue by Immunohistochemistry.
  • Anti-Hsp60 antibody (ab31115) at 1/25 dilution + lysate prepared from HeLa cells

    Predicted band size : 60 kDa

References for Anti-Hsp60 antibody (ab31115)

This product has been referenced in:
  • Jetzt AE  et al. Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome. Toxicol Appl Pharmacol 310:120-128 (2016). Read more (PubMed: 27639428) »
  • Agostini-Dreyer A  et al. Endogenous IGFBP-3 mediates intrinsic apoptosis through modulation of Nur77 phosphorylation and nuclear export. Endocrinology N/A:en20151215 (2015). Read more (PubMed: 26340041) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Sample Cow Cell lysate - whole cell (Normal mammary epithelial cells (nMEC))
Specification Normal mammary epithelial cells (nMEC)
Treatment 1.0 uM ANS +/- 100 ng/mL IGF-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"