The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Sequence similaritiesBelongs to the chaperonin (HSP60) family.
heat shock protein family D (Hsp60) member 1 antibody
Hsp 60 antibody
HSP 65 antibody
Mitochondrial matrix protein P1 antibody
P60 lymphocyte protein antibody
short heat shock protein 60 Hsp60s1 antibody
Anti-Hsp60 antibody images
Western blot - Anti-Hsp60 antibody (ab31115)This image is courtesy of an anonymous Abreview
All lanes : Anti-Hsp60 antibody (ab31115) at 1/8000 dilution
Lane 1 : Cow normal mammary epithelial cells (nMEC) treated with serum free media Lane 2 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin Lane 3 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1 Lane 4 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1 Lane 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media Lane 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin Lane 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1 Lane 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1
Lysates/proteins at 40 µg per lane.
Secondary ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 1/20000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 60 kDa Observed band size : 60 kDa
Exposure time : 15 seconds
This image is courtesy of an anonymous Abreview
Treatment for 30 minutes (lanes 1-4) or 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).
Ab31115, at a 1/200 dilution, staining Hsp60 in formalin fixed, paraffin embedded human colon carcinoma tissue by Immunohistochemistry.
Western blot - Hsp60 antibody (ab31115)
Anti-Hsp60 antibody (ab31115) at 1/25 dilution + lysate prepared from HeLa cells
Predicted band size : 60 kDa
References for Anti-Hsp60 antibody (ab31115)
This product has been referenced in:
Jetzt AE et al. Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome. Toxicol Appl Pharmacol310:120-128 (2016).
Read more (PubMed: 27639428) »
Agostini-Dreyer A et al. Endogenous IGFBP-3 mediates intrinsic apoptosis through modulation of Nur77 phosphorylation and nuclear export. EndocrinologyN/A:en20151215 (2015).
Read more (PubMed: 26340041) »