Overview

  • Product nameAnti-Hsp60 antibody
    See all Hsp60 primary antibodies
  • Description
    Rabbit polyclonal to Hsp60
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide mapping at the middle region of human Hsp60, identical to the related rat and mouse sequence.

Properties

Applications

Our Abpromise guarantee covers the use of ab68416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody images

  • ab68416 at 1µg/ml staining Hsp60 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by boiling. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • Anti-Hsp60 antibody (ab68416) at 0.5 µg/ml + Cell lysates prepared from Hela cells. at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 60 kDa

References for Anti-Hsp60 antibody (ab68416)

This product has been referenced in:
  • Deng MY  et al. Frontal-subcortical protein expression following prenatal exposure to maternal inflammation. PLoS One 6:e16638 (2011). WB ; Mouse . Read more (PubMed: 21347362) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"