Overview

  • Product nameAnti-Hsp60 antibody
    See all Hsp60 primary antibodies
  • Description
    Mouse polyclonal to Hsp60
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-573 of Human Hsp60 (NP_002147.2).

  • Positive control
    • Human kidney lysate, HepG2 lysate, Hsp60 transfected 293T cell lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Anti-Hsp60 antibody images

  • Anti-Hsp60 antibody (ab89609) at 1 µg/ml + Human kidney lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-Hsp60 antibody (ab89609) at 1 µg/ml + HepG2 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • All lanes : Anti-Hsp60 antibody (ab89609) at 1 µg/ml

    Lane 1 : Hsp60 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab89609 at 10 µg/ml staining Hsp60 in HeLa cells by Immunofluorescence.

References for Anti-Hsp60 antibody (ab89609)

ab89609 has not yet been referenced specifically in any publications.

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