RabMAb

Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Overview

  • Product nameAnti-Hsp60 antibody [EP1006Y] - Loading Control
    See all Hsp60 primary antibodies
  • Description
    Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the internal region of human Hsp60.

  • Positive control
    • T47D cell lysate, human breast carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

    Is unsuitable for FACS, ICC/IF or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
    • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
    • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein mitochondrial antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin antibody
      • Chaperonin, 60-KD antibody
      • CPN 60 antibody
      • CPN 60 antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • GroEL Homolog antibody
      • GroEL Homolog antibody
      • Heat shock 60kD protein 1 chaperonin antibody
      • Heat shock 60kD protein 1 chaperonin antibody
      • Heat shock 60kDa protein 1 antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat Shock Protein 60 antibody
      • Heat Shock Protein 60 antibody
      • Heat shock protein 65 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD 1 antibody
      • HSPD 1 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • P60 lymphocyte protein antibody
      • Short heat shock protein 60 Hsp60s1 antibody
      • Spastic paraplegia 13 antibody
      • SPG 13 antibody
      • SPG13 antibody
      • SPG13 antibody
      see all

    Anti-Hsp60 antibody [EP1006Y] - Loading Control images

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution

      Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
      Lane 3 : 293T (Human embryonic kidney) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 60 kDa

      Blocking/dilution buffer: 5% NFDM /TBST

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human fetal heart lysates
      Lane 3 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 60 kDa

      Blocking/dilution buffer: 5% NFDM /TBST

    • All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Rat brain lysates
      Lane 4 : Rat heart lysates
      Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 60 kDa
    • Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

      Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    • Flow cytometry analysis of 2% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma) cells labeling Hsp60 with ab45134 at 1/180 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is ab172730 rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

    References for Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

    This product has been referenced in:

    See 1 Publication for this product

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