Recombinant Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp60 antibody [EP1006Y] - Loading Control
See all Hsp60 primary antibodies -
Description
Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Hsp60 aa 400-500 (internal sequence).
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Positive control
- T47D cell lysate, human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1006Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 63868 Rat
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- SwissProt: P63039 Rat
- Unigene: 595053 Human
see all -
Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution
Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
Lane 3 : 293T (Human embryonic kidney) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDaBlocking/dilution buffer: 5% NFDM /TBST
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Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 60 kDaBlocking/dilution buffer: 5% NFDM /TBST
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Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat heart lysates
Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa -
Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab45134 has been referenced in 15 publications.
- Dörner K et al. Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. Nucleic Acids Res 50:2872-2888 (2022). PubMed: 35150276
- Niikura R et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663
- Li L et al. Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis. Sci Rep 12:11830 (2022). PubMed: 35821263
- Nebie O et al. Human platelet lysate biotherapy for traumatic brain injury: preclinical assessment. Brain 144:3142-3158 (2021). PubMed: 34086871
- Kim HJ et al. RORα Enhances Lysosomal Acidification and Autophagic Flux in the Hepatocytes. Hepatol Commun 5:2121-2138 (2021). PubMed: 34558854