RabMAb

Anti-Hsp60 [EP1006Y] antibody - Loading Control (ab45134)

Overview

  • Product nameAnti-Hsp60 [EP1006Y] antibody - Loading ControlSee all Hsp60 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
  • Tested applicationsIHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the internal region of human Hsp60.

  • Positive control
    • T47D cell lysate, human breast carcinoma tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab45134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
WB
  • Application notesIHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

    Is unsuitable for FACS, ICC/IF or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
    • Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
    • Sequence similaritiesBelongs to the chaperonin (HSP60) family.
    • Cellular localizationMitochondrion matrix.
    • Target information above from: UniProt accession P10809 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein mitochondrial antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin antibody
      • Chaperonin, 60-KD antibody
      • CPN 60 antibody
      • CPN 60 antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • GroEL Homolog antibody
      • GroEL Homolog antibody
      • Heat shock 60kD protein 1 chaperonin antibody
      • Heat shock 60kD protein 1 chaperonin antibody
      • Heat shock 60kDa protein 1 antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat Shock Protein 60 antibody
      • Heat Shock Protein 60 antibody
      • Heat shock protein 65 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD 1 antibody
      • HSPD 1 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • P60 lymphocyte protein antibody
      • Short heat shock protein 60 Hsp60s1 antibody
      • Spastic paraplegia 13 antibody
      • SPG 13 antibody
      • SPG13 antibody
      • SPG13 antibody
      see all

    Anti-Hsp60 [EP1006Y] antibody - Loading Control images

    • All lanes : Anti-Hsp60 [EP1006Y] antibody - Loading Control (ab45134) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 61 kDa (why is the actual band size different from the predicted?)


      Exposure time : 4 minutes

      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab45134 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Ab45134 at 1/100 staining human Hsp60 in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.

    References for Anti-Hsp60 [EP1006Y] antibody - Loading Control (ab45134)

    This product has been referenced in:

    See 1 Publication for this product

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