Hsp60 overexpression 293T lysate (whole cell) (ab94126)

Overview

  • Product nameHsp60 overexpression 293T lysate (whole cell)
  • Description
    Hsp60 overexpression lysate
  • General notesab94126 is a 293T cell transfected lysate in which Human Hsp60 has been transiently over-expressed using a pCMV-Hsp60 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDisease: Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Similarity: Belongs to the chaperonin (HSP60) family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Hsp60 overexpression 293T lysate (whole cell) images

  • ab94126 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Hsp60 antibody (ab89609) at 1/500 dilution

    Lane 1 : Hsp60 overexpression 293T lysate (whole cell) (ab94126)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Hsp60 overexpression 293T lysate (whole cell) (ab94126)

ab94126 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"