Overview

  • Product nameAnti-Hsp70 antibodySee all Hsp70 primary antibodies ...
  • Description
    Rabbit polyclonal to Hsp70
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Dog, Human, Fruit fly (Drosophila melanogaster), Fish
  • Immunogen

    Synthetic peptide derived from the sequence of Rat Hsp70.

  • Positive control
    • Mouse 3T3 and heat shocked 3T3 cell lysates; Purified Hsp70 protein and purified Hsc70 protein; Human Spleen neutrophils

Properties

Applications

Our Abpromise guarantee covers the use of ab109689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 70 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificityHSPA1B is testis-specific.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P08107 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DnaK type molecular chaperone HSP70 1 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HSP70 1 antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Anti-Hsp70 antibody images

  • All lanes : Anti-Hsp70 antibody (ab109689) at 1/1000 dilution

    Lane 1 : 3T3 cell lysate
    Lane 2 : Heat shocked 3T3 cell lysate
    Lane 3 : Purified Hsp70 protein
    Lane 4 : Purified Hsc70 protein


    Predicted band size : 70 kDa
  • ab109689, at 10 µg/ml, staining Hsp70 + Hsc70 in formalin-fixed, paraffin-embedded Human Spleen neutrophils by Immunohistochemistry.

References for Anti-Hsp70 antibody (ab109689)

ab109689 has not yet been referenced specifically in any publications.

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