The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). (ECL).
FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Tissue specificityHSPA1B is testis-specific.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Western blot - Anti-Hsp70 antibody [C96F3-3] (ab115651)
All lanes : Anti-Hsp70 antibody [C96F3-3] (ab115651) at 1/1000 dilution
Lane 1 : HSP70 Recombinant Human Protein Lane 2 : HSP70B’ Recombinant Human Protein Lane 3 : HSP70-A1 Recombinant Mouse Protein Lane 4 : HSP70 Recombinant Chinook Salmon Protein Lane 5 : HSC70 Recombinant Bovine Protein Lane 6 : HSC70 (ATPase Fragment) Recombinant Bovine Protein Lane 7 : HeLa Cell Lysate Lane 8 : HeLa Cell Lysate, Heat Shocked Lane 9 : 3T3 Cell Lysate Lane 10 : 3T3 Cell Lysate, Heat Shocked Lane 11 : PC-12 Cell Lysate Lane 12 : PC-12 Cell Lysate, Heat Shocked
Predicted band size : 70 kDa
References for Anti-Hsp70 antibody [C96F3-3] (ab115651)
has not yet been referenced specifically in any publications.
Publishing research using ab115651? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"