Overview

  • Product nameAnti-Hsp70 antibody [N27F34]
    See all Hsp70 primary antibodies
  • Description
    Mouse monoclonal [N27F34] to Hsp70
  • Tested applicationsWB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Pig, Xenopus laevis, Fish, Monkey, Plants
  • Immunogen

    Purified Hsc70 and Hsp70 isolated from Human HeLa cells.

  • Positive control
    • Hsc70 protein, HSP70 Protein, Human colon cancer Caco2 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab90347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Flow Cyt
IHC-P
  • Application notesFlow Cyt: 1/100.
    WB: 1/1000. Predicted molecular weight: 71 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Hsp70 antibody [N27F34] images

    • IHC image of Hsp70 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90347, 0.1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-Hsp70 antibody [N27F34] (ab90347) at 1/1000 dilution

      Lane 1 : Hsp70 protein
      Lane 2 : Hsc70 protein


      Predicted band size : 71 kDa
    • Human colon cancer Caco2 cells analyzed by Flow Cytometry using isotype control antibody (left) and ab90347 (right).

    References for Anti-Hsp70 antibody [N27F34] (ab90347)

    This product has been referenced in:
    • Wills J  et al. Paraquat, but Not Maneb, Induces Synucleinopathy and Tauopathy in Striata of Mice through Inhibition of Proteasomal and Autophagic Pathways. PLoS One 7:e30745 (2012). WB ; Mouse . Read more (PubMed: 22292029) »
    • Kabakov AE  et al. Stressful preconditioning and HSP70 overexpression attenuate proteotoxicity of cellular ATP depletion. Am J Physiol Cell Physiol 283:C521-34 (2002). WB . Read more (PubMed: 12107062) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your inquiry. I have confirmed with the lab that these are in fact the same antibodies. I have alerted our datasheets team and they will update the datasheets to reflect this. I have looked into this and have concluded that Hsp70 and Hsc7...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"