Anti-Hsp70 antibody [N27F34] (Alkaline Phosphatase) (ab69561)

Overview

  • Product nameAnti-Hsp70 antibody [N27F34] (Alkaline Phosphatase)See all Hsp70 primary antibodies ...
  • Description
    Mouse monoclonal [N27F34] to Hsp70 (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • Conjugation notesThis antibody was purified by ion exchange chromatography and subsequently conjugated to alkaline phosphatase using a bifunctional crosslinker.
  • SpecificityThis antibody detects 72 and 73 kDa proteins, corresponding to the apparent molecular masses of the inducible hsp70 and constitutive hsc70 respectively on SDS-PAGE immunoblots.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fish, Monkey
    Predicted to work with: Plants
  • Immunogen

    Purified Hsc70 and Hsp70 isolated from human HeLa cells.

  • EpitopeThe epitope is in the region of amino acids 503-640 of human Hsp70.
  • Positive control
    • HeLa Heat Shocked cell lysate. Recombinant Human Hsp70 Protein. Bovine Hsc70 Protein.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 150mM Sodium chloride, 100mM Tris, 1mM Magnesium chloride, 0.1 mM Zinc, pH 7.6
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Clonality Monoclonal
  • Clone numberN27F34
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 71 kDa.

Target

  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificityHSPA1B is testis-specific.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • Constitutive heat shock protein 70 antibody
    • dnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kD protein 1 antibody
    • Heat shock 70kD protein 10 (HSC71) antibody
    • Heat shock 70kD protein 8 (HSP73) antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock cognate 71 kDa protein antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1/HSP70 2 antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    • Lipopolysaccharide associated protein 1 antibody
    • LPS associated protein 1 antibody
    • MGC131511 antibody
    • MGC29929 antibody
    • N myristoyltransferase inhibitor protein 71 antibody
    • NIP71 antibody
    • Uncharacterized bone marrow protein BM034 antibody
    see all

References for Anti-Hsp70 antibody [N27F34] (Alkaline Phosphatase) (ab69561)

ab69561 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. I have confirmed with the lab that these are in fact the same antibodies. I have alerted our datasheets team and they will update the datasheets to reflect this. I have looked into this and have concluded that Hsp70 and Hsc7...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"