Overview

  • Product nameAnti-Hsp70 antibody [W27]
    See all Hsp70 primary antibodies
  • Description
    Mouse monoclonal [W27] to Hsp70
  • SpecificityRecognizes both the constitutive and inducible forms of Hsp70.
  • Tested applicationsWB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Cow, Human, Monkey
  • Immunogen

    Hsp70 protein from HeLa cells

  • Positive control
    • BT474 or HeLa cells. Breast carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from ascites fluid by Protein A chromatography.
  • Clonality Monoclonal
  • Clone numberW27
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab81334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    IP (native and denatured): Use at 2 µg/mg of lysate.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
    • Tissue specificityHSPA1B is testis-specific.
    • Sequence similaritiesBelongs to the heat shock protein 70 family.
    • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAQB 147D11.1 001 antibody
      • DnaK type molecular chaperone HSP70 1 antibody
      • Epididymis secretory protein Li 103 antibody
      • FLJ54303 antibody
      • FLJ54370 antibody
      • FLJ54392 antibody
      • FLJ54408 antibody
      • FLJ75127 antibody
      • Heat shock 70 kDa protein 1 antibody
      • Heat shock 70 kDa protein 1/2 antibody
      • Heat shock 70 kDa protein 1A/1B antibody
      • Heat shock 70kDa protein 1A antibody
      • Heat shock 70kDa protein 1B antibody
      • Heat shock induced protein antibody
      • Heat shock protein 70 antibody
      • HEL S 103 antibody
      • HSP70 1 antibody
      • HSP70 1B antibody
      • HSP70 2 antibody
      • HSP70-1/HSP70-2 antibody
      • HSP70-1A antibody
      • HSP70.1 antibody
      • HSP70.1/HSP70.2 antibody
      • HSP70I antibody
      • HSP71_HUMAN antibody
      • HSP72 antibody
      • HSPA1 antibody
      • HSPA1A antibody
      • HSPA1B antibody
      • XXbac BCX40G17.3 001 antibody
      see all

    Anti-Hsp70 antibody [W27] images

    • Formalin fixed, paraffin embedded human breast carcinoma stained with anti Hsp70 (form of ab81334 containing BSA and azide) at 4 µg/ml. Peroxidase conjugate and DAB chromogen were used. Note cytoplasmic and nuclear staining of tumor cells.
    • anti Hsp70 (form of ab81334 containing BSA and azide) at 2 µg/ml + human cells

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    References for Anti-Hsp70 antibody [W27] (ab81334)

    ab81334 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab81334.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"