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|Sample type||Average %||Range|
|Tissue Extracts||>=90||% - %|
|Cell Lysate||>=90||% - %|
Abcam’s HSP70 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in samples from Human, Mouse and Rat origins. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.
A HSP70 monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.
In most mammals, the expression of inducible HSP70 is strictly stress inducible and can only be detected following a significant stress upon the cell or organism. However, in humans and primates, inducible HSP70 is present at basal levels and is upregulated in response to stress. The role of HSP70 has been studied in a variety of medically relevant models or conditions such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 has also been studied in the normal processes of aging, spermatogenesis, menstruation, and physical activity such as exercise.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 100ml|
|5X Extraction Reagent||1 x 10ml|
|Anti-HSP70 Microplate (12 x 8 wells)||1 unit|
|Anti-rabbit IgG HSP70 Horseradish Peroxidase Conjugate||1 x 10ml|
|HSP70 Antibody||1 x 10ml|
|Recombinant HSP70 Standard||1 x 25µl|
|Sample Diluent 2||1 x 50ml|
|Stop Solution 2||1 x 10ml|
|TMB Substrate||1 x 10ml|
Our Abpromise guarantee covers the use of ab133060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|