Overview

  • Product name
    HSP70 ELISA Kit
    See all HSP70 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 24 < 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9 < 13%
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    200 pg/ml
  • Range
    780 pg/ml - 50000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts >=90 % - %
    Cell Lysate >=90 % - %

  • Assay time
    4h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s HSP70 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in samples from Human, Mouse and Rat origins. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.


    A HSP70 monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.


    Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA® range.

  • Notes

    Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.

     

    In most mammals, the expression of inducible HSP70 is strictly stress inducible and can only be detected following a significant stress upon the cell or organism. However, in humans and primates, inducible HSP70 is present at basal levels and is upregulated in response to stress. The role of HSP70 has been studied in a variety of medically relevant models or conditions such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 has also been studied in the normal processes of aging, spermatogenesis, menstruation, and physical activity such as exercise.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab133060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

HSP70 ELISA Kit images

  • Representative Standard Curve using ab133060.

Protocols

References for HSP70 ELISA Kit (ab133060)

This product has been referenced in:
  • Oraby AM  et al. Cognitive Function and Heat Shock Protein 70 in Children With Temporal Lobe Epilepsy. J Child Neurol N/A:N/A (2016). Sandwich ELISA ; Human . Read more (PubMed: 27664194) »
  • Choi JI  et al. Semi-quantitative analyses of hippocampal heat shock protein-70 expression based on the duration of ischemia and the volume of cerebral infarction in mice. J Korean Neurosurg Soc 55:307-12 (2014). Mouse . Read more (PubMed: 25237425) »

See all 2 Publications for this product

Product Wall

Unfortunately, we have not tested this kit on Neisseria meningitidis and Neisseria lactamica lysates. I have compared the total human Hsp70 protein sequence with the Neisseria meningitidis and Neisseria lactamica sequences. Unfortunately, there is onl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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