HSP70 ELISA Kit (ab133060)

Overview

• Product name
  HSP70 ELISA Kit
  See all HSP70 kits
• Detection method
  Colorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall	24			< 5%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall	9			< 13%

• Sample type
  Tissue Extracts, Cell Lysate
• Assay type
  Sandwich (quantitative)
• Sensitivity
  200 pg/ml
• Range
  780 pg/ml - 50000 pg/ml
• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Tissue Extracts	>=90	% - %
  Cell Lysate	>=90	% - %

• Assay time
  4h 30m
• Assay duration
  Multiple steps standard assay
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Product overview

  Abcam’s HSP70 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in samples from Human, Mouse and Rat origins. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.

  
  

  A HSP70 monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

  
  

  Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA^® range.

• Notes

  Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.

   

  In most mammals, the expression of inducible HSP70 is strictly stress inducible and can only be detected following a significant stress upon the cell or organism. However, in humans and primates, inducible HSP70 is present at basal levels and is upregulated in response to stress. The role of HSP70 has been studied in a variety of medically relevant models or conditions such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 has also been studied in the normal processes of aging, spermatogenesis, menstruation, and physical activity such as exercise.

• Tested applications
  Suitable for: Sandwich ELISAmore details
• Platform
  Microplate

Properties

• Storage instructions
  Please refer to protocols.

• Components	1 x 96 tests
  20X Wash Buffer Concentrate	1 x 100ml
  5X Extraction Reagent	1 x 10ml
  Anti-HSP70 Microplate (12 x 8 wells)	1 unit
  Anti-rabbit IgG HSP70 Horseradish Peroxidase Conjugate	1 x 10ml
  HSP70 Antibody	1 x 10ml
  Recombinant HSP70 Standard	1 x 25µl
  Sample Diluent 2	1 x 50ml
  Stop Solution 2	1 x 10ml
  TMB Substrate	1 x 10ml

• Research areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Signal transduction proteins ELISA kits

HSP70 ELISA Kit images

• 

  Typical Standard Curve

  Representative Standard Curve using ab133060.