Overview

  • Product nameanti-HSP70 IgG/A/M Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    sample 16 < 10%
  • Sample type
    Serum
  • Assay typeIndirect
  • Sensitivity
    6.79 ng/ml
  • Range
    31.25 ng/ml - 1000 ng/ml
  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-HSP70 IgG/A/M in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of anti-HSP70 antibodies in serum samples from human origin.

    A recombinant Human Hsp70 protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. An anti-Human GAM-HRP conjugated antibody is then added and incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

     

  • Notes

    The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

  • Tested applicationsIndirect ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • IgA
    • IgG
    • IgM
    • Immunoglobulin A
    • Immunoglobulin G
    • Immunoglobulin M
    see all

Applications

Our Abpromise guarantee covers the use of ab133063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

anti-HSP70 IgG/A/M Human ELISA Kit images

  • Representative Standard Curve using ab133063.

Protocols

References for anti-HSP70 IgG/A/M Human ELISA Kit (ab133063)

ab133063 has not yet been referenced specifically in any publications.

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