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Recombinant human Hsp90 (1-732aa) purified from E. coli.
Our Abpromise guarantee covers the use of ab48022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/100 - 1/200.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
ab48022 staining Hsp90 alpha in human urothelium tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB polymer detection system was used with a DAB chromogen.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HSP90 with ab48022 at 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Flow Cytometry analysis of HepG2 cells labelling HSP90 with ab48022, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.
ab48022 has not yet been referenced specifically in any publications.