Overview

  • Product nameAnti-Hsp90 alpha antibody
    See all Hsp90 alpha primary antibodies
  • Description
    Rabbit polyclonal to Hsp90 alpha
  • Specificityab74248 weakly cross reacts with Hsp90 beta
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Chimpanzee, Cynomolgus Monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Mouse Hsp90 alpha aa 2-12 (N terminal).
    Sequence:

    PEETQTQDQPM-Cys

  • Positive control
    • MAD109 cells or breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab74248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 85 kDa.

Use at a concentration of 5 - 10 µg/ml for 2 hours at room temperature.

IP Use at 10 µg/mg of lysate.

(Native verified, use Protein A)

IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at a concentration of 5 - 10 µg/ml for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 to 20 minutes followed by cooling at room temperature for 20 minutes.

ICC/IF Use at an assay dependent concentration.

Target

Anti-Hsp90 alpha antibody images

  • Formalin fixed, paraffin embedded human breast carcinoma tissue stained with ab74248 at 10µg/ml, using peroxidase conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of tumor cells.
  • Anti-Hsp90 alpha antibody (ab74248) at 10 µg/ml + Mouse cells-1

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
  • IP/Western blot of mouse cells-1. IP with ab74248 at 10µg/mg protein lysates.

References for Anti-Hsp90 alpha antibody (ab74248)

This product has been referenced in:
  • Wang H  et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). Read more (PubMed: 25359884) »

See 1 Publication for this product

Product Wall

We do carry a number of control lysates and tissue slides including those from breast tumor carcinoma. I have included links to these below. Breast tumor (human): ductal carcinoma (invasive) tissue slides (ab4697): http://www.abcam.com/breast-tumor-...

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Thank you for your reply. I can have ab79849 shipped to you next week but I want to make sure that there will be someone there to receive it. As it is being shipped to , we cannot guarantee a delivery date as there is a chance that it could get...

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Thank you for your reply. I am sorry about the problems that you are having with ab74248 in western blot. I would be happy to send you a replacement antibody, a few possible alternatives are: http://www.abcam.com/Hsp90-alpha-antib...

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Thank you for contacting Abcam. I am sorry that you are having issues with ab74248 in western blot. We do guarantee that this antibody will work in western blot in mouse, rat and human cells. I was wondering if you could please tell me which type of ce...

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Thank you for attending our webinar on Immunoprecipitation last week. We hope you enjoyed it and it was useful for you. We would also like to thank you for sending your question and apologize that we were not able to answer it in the Q&A session. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"