Overview

  • Product nameAnti-Hsp90 alpha antibody [D7a]
    See all Hsp90 alpha primary antibodies
  • Description
    Mouse monoclonal [D7a] to Hsp90 alpha
  • Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Pig
  • Immunogen

    Full length native protein (purified) (Chicken)

  • Positive control
    • HeLa (heat shocked) cell lysate; human Hsp90 alpha protein

Properties

Applications

Our Abpromise guarantee covers the use of ab59459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 95 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200.

Target

Anti-Hsp90 alpha antibody [D7a] images

  • ab59459 staining Hsp90 alpha in the THP-1 monocyte/macrophage cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.1% and blocked with 5% Serum for 60 minutes at 20°C. Samples were incubated with primary antibody (1/200 1% BSA/5% Goat Serum) for 1 hour at 20°C. An Alexa Fluor®647-conjugated Goat anti-mouse IgG polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Hsp90 alpha antibody [D7a] (ab59459) at 1/1000 dilution + Rat tissue lysates

    Predicted band size : 95 kDa
  • ab59459 staining Hsp90 alpha in human colon cancer tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave in citrate buffer. The primary antibody was used at dilution of 1/100,000 dilution using antibody amplifier™ system.

  • Overlay histogram showing HeLa cells stained with ab59459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59459, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Hsp90 alpha antibody [D7a] (ab59459)

This product has been referenced in:
  • Zhang Y  et al. A systems biology-based investigation into the therapeutic effects of Gansui Banxia Tang on reversing the imbalanced network of hepatocellular carcinoma. Sci Rep 4:4154 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24561634) »
  • Barabutis N  et al. LPS induces pp60c-src-mediated tyrosine phosphorylation of Hsp90 in lung vascular endothelial cells and mouse lung. Am J Physiol Lung Cell Mol Physiol 304:L883-93 (2013). Mouse, Human . Read more (PubMed: 23585225) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Apr 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1 moncyte/macrophage cell line)
Specification THP-1 moncyte/macrophage cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. James Harris

Verified customer

Submitted Aug 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"