Anti-Hsp90 alpha antibody [EPR3953] (ab109248)


  • Product name
    Anti-Hsp90 alpha antibody [EPR3953]
    See all Hsp90 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR3953] to Hsp90 alpha
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to region within Human Hsp90 alpha.

  • Positive control
    • WB: HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue and Human kidney tissue IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab109248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Molecular chaperone. Has ATPase activity.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Post-translational
    • Cellular localization
      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • EL52 antibody
      • epididymis luminal secretory protein 52 antibody
      • Heat shock 86 kDa antibody
      • heat shock 90kD protein 1, alpha antibody
      • Heat shock 90kD protein 1, alpha like 4 antibody
      • heat shock 90kD protein, alpha-like 4 antibody
      • Heat shock 90kDa protein 1 alpha antibody
      • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 86 antibody
      • HSP86 antibody
      • Hsp89 antibody
      • HSP89A antibody
      • Hsp90 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90ALPHA antibody
      • HSP90N antibody
      • HSPC1 antibody
      • HSPCA antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • HSPN antibody
      • LAP 2 antibody
      • LAP2 antibody
      • lipopolysaccharide-associated protein 2 antibody
      • LPS-associated protein 2 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Anti-Hsp90 alpha antibody [EPR3953] images

    • All lanes : Anti-Hsp90 alpha antibody [EPR3953] (ab109248) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in HeLa cells by Immunofluorescence.

    References for Anti-Hsp90 alpha antibody [EPR3953] (ab109248)

    This product has been referenced in:
    • Guo W  et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . Read more (PubMed: 24465691) »
    • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »

    See all 2 Publications for this product

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