Anti-Hsp90 alpha antibody [Hyb-K41009] (ab59464)

Overview

  • Product nameAnti-Hsp90 alpha antibody [Hyb-K41009]
    See all Hsp90 alpha primary antibodies
  • Description
    Mouse monoclonal [Hyb-K41009] to Hsp90 alpha
  • Specificityalpha-specific
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant human hsp90 alpha

  • Positive control
    • Heat shocked HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab59464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 95 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionMolecular chaperone. Has ATPase activity.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 alpha antibody [Hyb-K41009] images

  • ab59464 at 1/5000 dilution staining Hsp90 alpha in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A HRP-conjugated anti mouse was used at 1/10 dilution as secondary. An antibody amplifier™ system was used for staining.

  • ab59464 at 1/5000 dilution staining Hsp90 alpha in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A HRP-conjugated anti mouse was used at 1/10 dilution as secondary. An antibody amplify™ system was used for staining.

  • All lanes : Anti-Hsp90 alpha antibody [Hyb-K41009] (ab59464)

    Lane 1 : Molecular Weight Marker
    Lane 2 : Human Hsp90 alpha protein at 2 µg


    Predicted band size : 95 kDa

References for Anti-Hsp90 alpha antibody [Hyb-K41009] (ab59464)

ab59464 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"