The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Working dilution: Ready-to-use for 30 min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
The optimal dilution for a specific application should be determined by the investigator.
FunctionMolecular chaperone. Has ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 90 family.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.