Overview

  • Product name
    Anti-Hsp90 antibody [16F1]
    See all Hsp90 primary antibodies
  • Description
    Rat monoclonal [16F1] to Hsp90
  • Specificity
    ab13494 detects both the alpha and beta isoforms of Hsp90.
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Drosophila melanogaster, Monkey, Zebrafish, Rainbow trout
    Predicted to work with: Plants
  • Immunogen

    Human Hsp90 purified from therapeutic orchiectomy specimens.

  • Positive control
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
  • General notes

    This antibody is highly recommended over other Hsp90 antibodies for use with samples of fish origin.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab13495 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This product has been affinity purified.
  • Clonality
    Monoclonal
  • Clone number
    16F1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
ELISA 1/5000 - 1/25000.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
IP Use at an assay dependent dilution.

Target

Images

  • Lane 1 : Anti-Hsp90 antibody [16F1] (ab13494) at 1000
    Lane 2 : No primary

    Lane 1 : 15ug/lane HEK293 whole cell lysate
    Lane 2 : 15ug/lane HEK293 whole cell lysate

    Secondary
    Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Alan Hendrick

    See Abreview

  • All lanes : Anti-Hsp90 antibody [16F1] (ab13494) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Hsp90 native protein
    Lane 3 : Hsp90 recombinant protein
    Lane 4 : Hsp90 alpha recombinant protein
    Lane 5 : heat shocked Hela cell lysates
    Lane 6 : L-929 cell lysates
    Lane 7 : Rat-2 cell lysates
    Lane 8 : RK-13 cell lysates


    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa

References

This product has been referenced in:
  • Tawo R  et al. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover. Cell 169:470-482.e13 (2017). Read more (PubMed: 28431247) »
  • Jørgensen MM  et al. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 4:26048 (2015). Read more (PubMed: 25862471) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mouse Cell lysate - whole cell (primary embryonic neurons)
Specification
primary embryonic neurons
Blocking step
Amersham blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2014

Thank you for contacting us. To our knowledge, tha antibody ab13494 has not been tested in flow cytometry. It may work for that application, on fixed and permeabilized samples, but we do not guarantee this. We do not have small samples for testing....

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I am very pleased to hear you would like to accept our offer and test ab13494 in fall armyworm (SF9 cells). This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for fall armyworm a...

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Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Tissue sections (Larval CNS)
Specification
Larval CNS
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0.1% triton
Username

Abcam user community

Verified customer

Submitted Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (aortic endothelial)
Loading amount
5 µg
Specification
aortic endothelial
Treatment
Immunoprecipitated with AMPK
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
20 µg
Specification
Lung
Treatment
TSA 1uM
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293 Cell Line)
Loading amount
15 µg
Specification
HEK-293 Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Dr. Alan Hendrick

Verified customer

Submitted Oct 23 2006

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab13494. I think the fact that another antibody against Hsp90 worked well rules out the possibility that your samples express low levels of the ...

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Thank you for your enquiry. I have added references for the antibody as used in IHC to the datasheet. You may refer to these articles for further information. I have included a link to the datasheet below for your convenience. I hope this infor...

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1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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