Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp90
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide designed between amino acids 250-325 of Human Hsp90.

  • Positive control
    • Lysate from heat shocked HeLa, L-929 or Rat-2 cells. IHC: Human placenta, prostate or small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab109704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-Hsp90 antibody (ab109704) at 1/1000 dilution

    Lane 1 : Heat shocked HeLa cell lysate
    Lane 2 : Heat shocked L-929 cell lysate
    Lane 3 : Heat shocked Rat-2 cell lysate


    Predicted band size : 83 kDa
  • ab109704 at 10 µg/ml staining Hsp90 in formalin-fixed, paraffin-embedded tissue Human placenta tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    The slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109704 at 10 µg/ml staining Hsp90 in formalin-fixed, paraffin-embedded tissue Human prostate tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    The slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab109704 at 10 µg/ml staining Hsp90 in formalin-fixed, paraffin-embedded tissue Human small intestine tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
    The slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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