Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp90
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 250-325 of Human Hsp90

  • Positive control
    • Heat Shocked HeLa, 3T3 and Rat 2 Cell Lysates IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab90555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    WB: 1/1000. Predicted molecular weight: 90 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • ICC/IF image of ab90555 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-Hsp90 antibody (ab90555) at 1/1000 dilution

      Lane 1 : Molecular weight marker
      Lane 2 : HeLa Cell Lysate (Heat Shocked)
      Lane 3 : 3T3 Cell Lysate (Heat Shocked)
      Lane 4 : Rat 2 Cell Lysate (Heat Shocked)


      Predicted band size : 83 kDa

    References

    This product has been referenced in:
    • Goldlust IS  et al. Mouse model implicates GNB3 duplication in a childhood obesity syndrome. Proc Natl Acad Sci U S A 110:14990-4 (2013). Read more (PubMed: 23980137) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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