Anti-Hsp90 antibody [AC-16] (ab6536)

Overview

  • Product name
    Anti-Hsp90 antibody [AC-16]
    See all Hsp90 primary antibodies
  • Description
    Mouse monoclonal [AC-16] to Hsp90
  • Specificity
    The antibody is reactive with both the constitutive and the inducible forms of HSP90. However, it does not bind to the native form of HSP90. It does not recognize HSP90 from E. coli and yeast.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Full length native protein (purified). (Achlya ambisexualis)

  • Positive control
    • HeLa lysate
  • General notes
    Monoclonal Anti-Heat Shock Protein 90 (mouse IgG2b isotype) is derived from the AC-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from B1OS mice immunized with heat shock protein 90 purified from the water mold Achlya ambisexualis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AC-16
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 83 kDa.

Target

Anti-Hsp90 antibody [AC-16] images

  • ab6536 at 1/2000 dilution staining Hsp90 in human colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer.

  • ab6536 at 1/2000 dilution staining Hsp90 in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. A antibody amplifier™ was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

References for Anti-Hsp90 antibody [AC-16] (ab6536)

ab6536 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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