Recombinant
RabMAb

Anti-Hsp90 antibody [EPNCIR102] (ab108593)

Overview

  • Product name
    Anti-Hsp90 antibody [EPNCIR102]
    See all Hsp90 primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR102] to Hsp90
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hsp90.

  • Positive control
    • WB: Hsp90 alpha and beta recombinant proteins and HeLa, Jurkat, SKBR3 and K562 cell lysates. IHC-P: Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Len Neckers. View antibodies from NCI Center for Cancer Research Collaboration.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Domain
      The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
    • Post-translational
      modifications
      ISGylated.
      S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
    • Cellular localization
      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock 86 kDa antibody
      • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
      • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
      • Heat shock protein HSP 90 alpha antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 84 antibody
      • HSP 86 antibody
      • Hsp 90 antibody
      • HSP86 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90AB1 antibody
      • HSP90B antibody
      • HSPC1 antibody
      • HSPC2 antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Images

    • All lanes : Anti-Hsp90 antibody [EPNCIR102] (ab108593) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SKBR3 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 85 kDa
    • ab108593 at 1/50 dilution staining Hsp90 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    • All lanes : Anti-Hsp90 antibody [EPNCIR102] (ab108593) at 1/500 dilution

      Lane 1 : Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
      Lane 2 : Recombinant human Hsp90 beta protein (ab80033)

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and dilution buffer: 5% NFDM/TBST.

    References

    ab108593 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108593.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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